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[upbeat electronic music...]

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[music fades]

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PRESENTER: The materials you will need are[br]one conventional, non-GM soybean.

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One GM soybean.

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Two reaction vials [br]or one microliter micro tube.

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Two transfer pipettes.

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Two lateral flow test strips.

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1% PBS buffer or distilled water.

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Four weigh boats.

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Access to tweezers, scoopulas,[br]paper towels, hammer,

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and isopropyl alcohol wipes.

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Using the seeds[br]in the GM soybean seed kit,

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allow students to observe and compare[br]the seeds.

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Students should look for observable[br]differences in shape, size, and color.

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Note: Little to no difference[br]will be observable in the seeds.

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As students observe,

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be sure that the two varieties[br]are kept separate.

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Explain to the class[br]that although the two seeds look the same,

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there is a difference between them

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and we are going to do an experiment[br]to try and discover it.

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Complete the following lab tests[br]for the class to see.

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Label the micro tubes[br]for identification of the seed

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that will be tested[br]by labeling one "A", and the other "B".

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Break the seed by placing one GM soybean

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between two small weight boats[br]and tapping it with a hammer.

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The seed should break into 2 or 3 pieces

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to allow enough[br]surface area to be exposed for extraction.

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Note: Do not crush the seed.

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Crushing can cause issues[br]recovering all pieces for extraction

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and may cause cross-contamination[br]of the testing area.

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Repeat this step[br]with the conventional soybean

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using separate weigh boats.

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Remove the top weigh boats

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and place the seed pieces[br]in the correct micro tube.

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If the seed is stuck to the boat,[br]use tweezers or a scoopula

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to gently release it.

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Do not touch the seeds with your hands[br]to avoid cross-contamination.

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Clean tweezers with[br]70% ethanol or isopropyl alcohol wipes

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to prevent cross-contamination.

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If any of the particles [br]of the GM seed sample

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end up in the conventional sample,

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you will get a false positive[br]on the test strip.

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So, it is important to use[br]different weigh boats

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and to clean the scoopula

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to prevent what we call in the lab,[br]"cross-contamination".

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Use the pipette to fill the micro tube[br]with 1% PBS buffer or distilled water.

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Approximately 0.5 milliliters

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Using the pipette as a pestle[br]and the micro tube as a mortar,

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stir in the seed pieces[br]for about 20 to 30 seconds.

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Be sure to stir with separate pipettes[br]to avoid cross-contamination.

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Let the micro tube with the pieces of seed[br]and distilled water or 1% PBS buffer,

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stand for 3 to 5 minutes.

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Place one lateral flow test strip[br]inside each vial

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with the arrow pointing downward.

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Allow the test to continue

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in the micro tube[br]at room temperature for five minutes.

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During this time, explain how[br]the lateral flow strip test works

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using the lesson plan.

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Interpreting the results.

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If the sample contains the CP for protein,[br]a second line will develop.

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[upbeat electronic music]

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[music fades]

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Note: If you grow the plants out,[br]you can also use the leaf tissue as well

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instead of the seeds.

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Just take a micro tube,[br]sandwich it between the cap and the leaf.

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Punch a small hole.

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Add your PBS buffer[br]or your distilled water.

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Use this as a mortar and pestle,[br]crush it up,

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and do the lateral flow strip test.

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Is there a difference[br]between these two varieties of soybean?

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Guide a class discussion[br]for students to conclude

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that these two varieties of soybean[br]are fundamentally the same.

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They are both soybeans.

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However, one variety has a protein[br]that the other does not.

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Ask students,[br]"How did the seed obtain that protein?"

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Introduce the concept

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that the genome of a plant can be changed[br]using a variety of methods

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In the following lesson,[br]students will learn

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how these two seed varieties [br]became different

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and explore[br]the impact that a single gene can have.

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[Outro electronic music...]