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[upbeat electronic music...]

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[music fades]

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PRESENTER: The materials you will need are
one conventional, non-GM soybean.

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One GM soybean.

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Two reaction vials 
or one microliter micro tube.

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Two transfer pipettes.

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Two lateral flow test strips.

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1% PBS buffer or distilled water.

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Four weigh boats.

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Access to tweezers, scoopulas,
paper towels, hammer,

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and isopropyl alcohol wipes.

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Using the seeds
in the GM soybean seed kit,

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allow students to observe and compare
the seeds.

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Students should look for observable
differences in shape, size, and color.

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Note: Little to no difference
will be observable in the seeds.

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As students observe,

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be sure that the two varieties
are kept separate.

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Explain to the class
that although the two seeds look the same,

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there is a difference between them

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and we are going to do an experiment
to try and discover it.

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Complete the following lab tests
for the class to see.

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Label the micro tubes
for identification of the seed

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that will be tested
by labeling one "A", and the other "B".

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Break the seed by placing one GM soybean

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between two small weight boats
and tapping it with a hammer.

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The seed should break into 2 or 3 pieces

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to allow enough
surface area to be exposed for extraction.

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Note: Do not crush the seed.

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Crushing can cause issues
recovering all pieces for extraction

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and may cause cross-contamination
of the testing area.

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Repeat this step
with the conventional soybean

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using separate weigh boats.

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Remove the top weigh boats

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and place the seed pieces
in the correct micro tube.

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If the seed is stuck to the boat,
use tweezers or a scoopula

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to gently release it.

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Do not touch the seeds with your hands
to avoid cross-contamination.

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Clean tweezers with
70% ethanol or isopropyl alcohol wipes

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to prevent cross-contamination.

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If any of the particles 
of the GM seed sample

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end up in the conventional sample,

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you will get a false positive
on the test strip.

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So, it is important to use
different weigh boats

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and to clean the scoopula

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to prevent what we call in the lab,
"cross-contamination".

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Use the pipette to fill the micro tube
with 1% PBS buffer or distilled water.

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Approximately 0.5 milliliters

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Using the pipette as a pestle
and the micro tube as a mortar,

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stir in the seed pieces
for about 20 to 30 seconds.

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Be sure to stir with separate pipettes
to avoid cross-contamination.

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Let the micro tube with the pieces of seed
and distilled water or 1% PBS buffer,

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stand for 3 to 5 minutes.

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Place one lateral flow test strip
inside each vial

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with the arrow pointing downward.

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Allow the test to continue

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in the micro tube
at room temperature for five minutes.

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During this time, explain how
the lateral flow strip test works

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using the lesson plan.

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Interpreting the results.

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If the sample contains the CP for protein,
a second line will develop.

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[upbeat electronic music]

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[music fades]

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Note: If you grow the plants out,
you can also use the leaf tissue as well

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instead of the seeds.

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Just take a micro tube,
sandwich it between the cap and the leaf.

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Punch a small hole.

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Add your PBS buffer
or your distilled water.

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Use this as a mortar and pestle,
crush it up,

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and do the lateral flow strip test.

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Is there a difference
between these two varieties of soybean?

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Guide a class discussion
for students to conclude

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that these two varieties of soybean
are fundamentally the same.

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They are both soybeans.

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However, one variety has a protein
that the other does not.

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Ask students,
"How did the seed obtain that protein?"

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Introduce the concept

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that the genome of a plant can be changed
using a variety of methods

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In the following lesson,
students will learn

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how these two seed varieties 
became different

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and explore
the impact that a single gene can have.

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[Outro electronic music...]